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The Confocal pressure myograph is a system specifically for use with laser scanning confocal microscopes (LSCM) and other high magnification imaging studies where intracellular processes within the smooth muscle cells or endothelial cells of the intact pressurized vessel wall are studied. Intracellular ion concentration such as calcium and trafficking of fluorescent probes, tags or proteins can be studied in this system, designed for small vessels (diameter >60μm).

easy access for high magnification/high numerical aperture lenses used in inverted microscopes

The conical open bath design allows easy access for high magnification/high numerical aperture lenses used in inverted microscopes. It also allows access for direct immersion lenses used in upright confocal microscopy. Furthermore, the top part of the myograph can be tipped, which facilitates maintenance and eases the mounting procedure.

Using angled custom mounting supports designed specifically to allow very precise vertical positioning, an isolated blood vessel can be positioned directly above or on top of the chamber window permitting use of lenses with working distances as low as 100 μm on an inverted LSCM.

The intravascular pressure and the pressure gradient along the vessel can be controlled precisely and longitudinal force in the segment measured continuously. A heating system with electronic feedback maintains bath temperature above ambient temperature. The customized chamber cover includes ports for superfusion, for rapid draining and filling and for gas supply. To facilitate cleaning, the chamber is an acid-resistant stainless steel.

When using the myograph on a confocal microscope, data acquisition of in- and outlet pressure, force and temperature is possible. This is achieved by either using the analog outputs or the serial interface.