The same techniques for implantation of Transonic Systems' flowprobes in rats are applied to vessel isolation and probe placement in the mouse, but on a smaller scale. Surgeries on this scale are performed with the aid of a surgical microscope
Measuring Flow in the Mouse Aorta T.L. Smith, PhD, Dept. of Orthopedics, Wake Forest University School of Medicine
To obtain the detailed procedural video showing step-by-step surgery for implantation of the Transonic 1.5PSL flowprobe on the ascending aorta of the mouse To order call: Tel: 607-257-5300; Fax 607-257-7256 See video stills and implantation protocol below
Implantation of the 1.5 SL Flowprobe on the Mouse Ascending Aorta
Visualization of ascending aorta in 1.5SL probe lumen.
1.5PSL Flowprobe positioned for closure
| Right Thoracotomy
Under anesthesia and after the mouse has been properly ventilated on a respirator, a right thoracotomy is performed in the 3rd intercostal space to expose the lungs in the thoracic cavity. The right lung is packed clear of the surgical field with a small piece of surgical sponge. The ascending aorta lies directly under the thymus gland.
Careful dissection of the ascending aorta is performed by blunt dissection using microsurgical vessel dilators to free the vessel from connective tissue. The vessel can be manipulated most easily by grasping the small fat pad at the base of the pulmonary artery.
After the aorta has been isolated from the pulmonary artery, two pieces of surgical silk are passed under the vessel to aid in placing the vessel within the lumen of the probe. The flowprobe is introduced into the thoracic cavity and the vessel lifted into the probe lumen so that the J-shaped reflector encircles the aorta.
After confirmation of vessel placement, the sutures are removed and the 1.5SL flowprobe is rotated so the probe cable exits laterally to the right. The thoracotomy can be closed over the probe to establish negative pressure in the thoracic cavity and improve venous return & cardiac output.
For acute measurements, an acoustic coupling gel injected into the flowprobe lumen is used to transmit the ultrasound signal. Gel is not required for chronic applications; fibrous tissue encapsulation of the flowprobe will provide good signal transmission after the animal recovers (3-5 days) and is ready for measurement & experimentation. |