心血管研究

代謝分析

生化分析/血球計數

生理訊號紀錄

生理教學

血流血氧

其他

呼吸研究

疼痛測試

神經科學

動物行為分析

動物照護設備

細胞

透析膜

無線遙測

給藥 / 採樣

試劑 / 耗材

運動生理

電生理

實驗器械

離體灌流系統

電生理

產品名稱：離子通道電生理應用 Ion Channel Eletrophysiology
產品編號：Ion Channel Eletrophysiology
廠　　牌：Multi Channel Systems

Ion Channel Eletrophysiology

The RoboocyteR system is a fully-automated all-in-one solution for high-throughput screenings based on the standard Xenopus oocytes expression system using the two-electrode voltage-clamp technique (TEVC).

We would like to show you here, how easy it is to setup an experiment with the RoboocyteR. Handling does not require special skills or special equipment. Look and see the movies for a quick overview and study the tutorials describing more detailed the use of the system from preparing the oocytes to analyzing the recorded data.

Oocyte preparation

In the oocyte preparation tutorial you will find all informations you need about all steps from removing the oocytes to selecting good ones, plating and washing them- of course including supply sources for needed additional materials.

The injection tutorial describes in detail preparing and starting an injection of your oocytes.

See also the movie about the preparation and the injection of the oocytes:

Recording and data analysis

The impalement of the oocytes for recording is fast, precise, and gentle, so minimizing cell damage. Repeated impalements of a single oocyte are possible.

See in the movie the RoboocyteR operating in the recording mode and the basic features of the data analysis:

Application Notes

Sodium Pump

The Na/K-ATPase or Na-pump is the pharmacological receptor for cardiac glycosides, which are widely used in the treatment of heart failure. Furthermore, it is possibly also the physiological receptor for endogenous ouabain compounds.

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GABAA Receptors

GABAA receptors are targets for many clinically important drugs like anxiolytics, anticonvulsants, anesthetics, sedatives, muscle relaxants, barbiturates, and benzodiazepines.

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IKs Currents (KCNE1)

Mutations in these ion channels lead to disorders that may increase the risk of death from cardiac arrhythmia (long QT syndrome or LQTS) and Jervell and Lange-Nielsen syndrome, associated with congenital deafness.

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nACh Receptors

nAChRs play a key role in the signal transmission between cells at the nerve/muscle synapses. Various studies demonstrated their involvement in high brain functions and in important neurodegenerative pathologies.

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Application Notes

P2X Receptors

P2X receptors are nucleotide-gated cation channels playing important physiological and pathophysiological roles and are therefore promising targets for drug development.

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Kv4.3 K+ Channels

Kv4.3 channels display rapidly inactivating K+ currents in brain and heart, which regulate neuronal firing and heart pacing. Altered Kv4.3 expression occurs under pathological conditions like heart failure.

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Cardiac Na+ Channels

These voltage-gated channels initiate action potentials in excitable cells. Local anesthetics such as lidocaine block Na+ channels and have been used to manage cardiac arrhythmias, and non-specific drug binding to the SCN5A channel can alter normal cardiac rhythm.

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hERG Channels

hERG channels critically contribute to cardiac repolarization. Drugs blocking these channels can lead to a prolongation of the action potential. Also, inhibition of hERG due to unwanted drug side effects means a significant risk factor for cardiac safety.

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Related products

http://www.multichannelsystems.com/applications/ion-channel-electrophysiology.html

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