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產品名稱:Paired Pulse Facilitation (PPF) or Depression (PPD)
產品編號:Paired Pulse Facilitation (PPF) or Depression (PPD
廠  牌:Multi Channel Systems

In Paired Pulse Facilitation (PPF) or Depression (PPD) experiments, the stimulus is followed by a second stimulus, typically 20-80 ms later. The magnitude of the population response to the second stimulus is compared to that of the first one. It is higher in case of a PPF, or lower in case of a PPD.

In MC_Rack, two (or more) regions of interest (ROI) can be defined, one triggered by the first stimulus, the other by the second stimulus, for example. The peak-to-peak amplitudes for both ROIs can be extracted online.

The example data file was recorded from a 300 μm thick acute hippocampus slice that was prepared from a 32 days old rat. The slice was stimulated with a single biphasic pulse (2 V amplitude, 100 μs per phase) by one of the embedded MEA-electrodes (No. 64). The second stimulus pulse was applied 20 ms following the first one. Recording was triggered by applying the SyncOut signal of the stimulus generator to one of the analog input channels (A1). Recorded sweeps are 200 ms, with a pre-trigger time of 30 ms, and a post-trigger time of 170 ms.

As the data was acquired with a MEA1060-BC amplifier with blanking circuit, all MEA electrodes were disconnected from the amplifier shortly during stimulation, in this case for 540 μs (300 μs Sync Out pulse of the STG, plus intrinsic Wait of 40 μs, plus user-defined Wait of 200 μs). This efficiently prevented stimulus artifacts.

Some electrodes recorded PPF and others PPD in this demo data file.


 

The demo data used was kindly provided by Dr. Frank Hofmann, University of Heidelberg, Germany.

 

Note:

In the downloadable tutorial, you will set up an offline analysis rack (for the demo data file) step by step. You can set up a virtual rack for online recordings and analysis likewise, simply add the MC_Card as the data source to the rack instead of the Replayer.

http://www.multichannelsystems.com/applications/neuronal-electrophysiology/paired-pulse-facillitation-ppf-or-depression-ppd.html

     
 
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